Journal article
Phenotypical and functional profiles of natural killer cells exhibiting matrix metalloproteinase-mediated CD16 cleavage after anti-HIV antibody-dependent activation
CC Tang, G Isitman, J Bruneau, C Tremblay, NF Bernard, SJ Kent, MS Parsons
Clinical and Experimental Immunology | WILEY-BLACKWELL | Published : 2015
DOI: 10.1111/cei.12593
Abstract
Natural killer (NK) cell-mediated antibody-dependent cellular cytotoxicity (ADCC) has been linked to protection from HIV infection and slower progression towards AIDS. However, antibody-dependent activation of NK cells results in phenotypical alterations similar to those observed on NK cells from individuals with progressive HIV infection. Activation of NK cells induces matrix metalloproteinase (MMP)-mediated cleavage of cell surface CD16. In the present study we assessed the phenotype and functional profile of NK cells exhibiting post-activation MMP-mediated CD16 cleavage. We found that NK cells achieving the highest levels of activation during stimulation exhibit the most profound decrease..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
We acknowledge Rachel Bouchard and Stephanie Matte for co-ordinating the cohorts of HIV-uninfected and HIV-infected slow progressors utilized for NK cell phenotyping studies. The following physicians are acknowledged for recruiting and following HIV-infected slow progressors: Dr Danielle Rouleau, Dr Colin Kovacs, Dr Mona Loutfy, Dr Marianne Harris, Dr Benoit Trottier, Dr Jean-Pierre Routy, Dr Anita Rachlis, Dr Pierre Cote, Dr Marian Klien, Dr Bertrand Lebouche, Dr Alan Piche, Dr Kenneth Logue and Dr Edward Ralph. Finally, we would like to acknowledge the study participants. This work was supported by programme grant number 510448 from the National Health and Medical Research Council (NHMRC), as well as grants from the Canadian Institutes for Health Research (CIHR) numbers HOP-123800, MOP-111155 and THA-118628 and the Fonds de la Recherche du Quebec Sante (FRQ-S) AIDS and Infectious Diseases Network. G. I. is the recipient of a Canadian HIV Trials Network postdoctoral fellowship. N. F. B. is a member of the Research Institute of the McGill University Health Centre, an institution funded in part by the FRQ-S. M. S. P. is the recipient of a CIHR postdoctoral fellowship.